Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...

With the launch of its new ProbeLibrary kits, Danish biotech firm Exiqon http://www.exiqon.com seeks to simplify real-time PCR. Typically 30 to 35 bases long, PCR ...

Performing Real-Time qPCR with the 5’ fluorogenic nuclease assay and a Double-Dye oligonucleotide probe provides additional specificity during PCR amplification, an essential component for a ...

The real-time polymerase chain reaction( PCR) has become a key tool for molecular assay development, expanding into applications as diverse as in vitro diagnostics (IVDs), food safety testing, and ...

Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...

Philadelphia, March 17, 2022 – After the start of the SARS-C0V-2 pandemic, investigators from ResearchPath LLC and their collaborators at Rutgers University quickly dedicated resources to develop ...

This is a preview. Log in through your library . Abstract Polymerase chain reaction (PCR) was used to produce biotinylated DNA probes for human papillomavirus (HPV) types 16 and 18. The specificity ...

The most common molecular assays rely on identifying the viral genomic material from a clinical specimen. In general, there are three important steps to this process that must be addressed for an ...

As SARS-C0V-2 variants emerged, researchers developed a PCR test that uses molecular beacons not only to diagnose COVID-19 infection, but also to identify the specific variant causing that infection.